Objective: These are the dynamic observation studies of 59Fe's obsorbed and / or transported metabolism course of the rat of deficient spleen syndrome by the application of radioactivity label technology on basement of both of pharmacokinetics and pharmacodynamics, and the influence of Fe transferrin receptor-mRNA expressed to the rat of deficient spleen syndrome by using UNA's spot hybridization technical. As well as, these are also the dynamic observation studies of 59Fe's obsorbed and / or transported metabolism course of the rat of deficient spleen syndrome, and the influence of Fe transferrin receptor-mRNA expressed to the rat of deficient spleen syndrome after Replenishing Spleen Method have been used. The experiment studies the relation of the spleen being the transferring owner or the spleen losing the transferring owner with Fe's metabolism, researches it's biology meaning of spleen being the owner of transmission, researches the science intension of important traditional Chinese medicine theory of spleen being the owner of transmission and the spleen serving as basement of the day after tomorrow, and provides the experiment basis of spleen merit ability and / or essence of deficient spleen syndrome.Methods: From the experiment I to the experiment V,the layer upon layer is gone deep into , the experimental research of the influence of 59Fe's metabolism and Fe transferrin receptor-mRNA expressed to the rat of deficient spleen syndrome by Replenishing Spleen Method.The experiment I: 80 male SD rats were randomly divided into 8 groups: normal group, normal replenishing spleen group, deficient spleen group, deficient spleen- replenishing spleen group, deficient Fe group, deficient Fe- replenishing spleen group, deficient Fe and spleen group, deficient Fe and spleen- replenishing spleen group. According to the requirement of the animal model standardizes most of rats were made the pattern. 8 groups were once irrigated shui xuanye of 59FeCl3 0. 2ml/220g/a rat. At the same time, according to weight of a person and a rat , 4 groups of normal replenishing spleen group, deficient spleen- replenishing spleen group, deficient Fe- replenishing spleen group, deficient Fe and spleen- replenishing spleen group were given 1. 5m± medicinal ingredients liquid of 1:1 four-man decoction, the others were given 1.5m± physiological saline , once a day. Respectively at 0. 5h , 1h , 1. 5h , 3h , 6h , 12h and 24h, blood 0. 2ml of all rats were taken from rat's tail,measured its radioactivity 59Fe with radiation immunity counter, to observe changes of pharmacokinetics of "Fe passing through rat's stomach and bowel way into the whole body blood-system.From the experiment II To the experiment V: 160 male SD rats were randomly divided into 8 groups: normal group, normal replenishing spleen group, deficient spleen group, deficient spleen- replenishing spleen group, deficient Fe group, deficient Fe- replenishing spleen group, deficient Fe and spleen group, deficient Fe and spleen- replenishing spleen group. According to the requirement of the animal model standardizes most of rats were made the pattern. 8 groups were once irrigated shui xuanye of 59FeCl3 0.2ml/220g/a rat. At the same time, according to weight of a person and a rat , 4 groups of normal replenishing spleen group, deficient spleen- replenishing spleen group, deficient Fe-replenishing spleen group, deficient Fe and spleen- replenishing spleen group were given 1. 5m眒edicinal ingredients liquid of 1:1 four-man decoction, the others were given 1. 5m眕hysiological saline , once a day. Respectively at 0. 5d 1d 2d 4d 8d and 14d 3 rats were chosen at random and killed in eyeball bloodletting law. All rats' organs of spleen , liver , small intestine , stomach and kidney were taken out and reserved for using: The experiment II all rats' organs of spleen , liver , small intestine , stomach and kidney were measured their radioactivity "Fe with y -radiation immunity counter, to observe changes of pharmacokinetics of transferring 59Fe from the whole body blood-sys...
|