The Study Of The Effect Of Electro-Acupuncture On The Learning And Memory Signal Transduction Mechanism And Brain Biochemistry In Laboratory Rats With Senile Dementia

Objective: To explore the mechanism of electro-acupuncture effect on senile dementia rat's learning and memory function.Methods: Divide senile dementia AD rats into normal control group, sham-operation group, model group and electro-acupuncture group randomly, adopt D-galactoside combinated aluminum chloride damage model, treat Baihui, Dazhui, Taixi and Zusanli with electro-acupuncture. Use biochemical method to observe the effect of electro-acupuncture on AchE, SOD, MACK NO, NOS of senile dementia AD rat's brain tissue; use immunohistochemistry technique to observe protein kinase expression of hippocampal; use optical and electron microscope to observe morphosis change of rat's brain tissue; use in situ hybridism technique to observe aspartate receptor mRNA expression of hippocampal; use water and electric maze experiment to observe rat escape latent period and mistake times, to understand it's learning and memory ability.Results: Electro-acupuncture can decrease AchE ability of VD rat's brain tissue evidently; electro-acupuncture can increase ability of SOD , inhibit ability of MAO , increase ability of antloxidation system; electro-acupuncture can increase model rat's NO, NOS content of hippocampal homogenate, increase learning and memory ability of rat ; electro-acupuncture can influence formation of long-term potentiation ( LTP) by regulate protein kintasc, aspartate receptor mRNA expression. Electro-acupuncture can influence morphosis of hippocampal synapse. Electro-acupuncture can decrease AD rat escape latent period and mistake times in water and electric maze experiment, improve AD rat's learning and memory in some extent. Electro-acupuncture is a simple and effective method for treating VD.