| As reports, the mortality of human body caused by prostate cancer has been stood the second place among all diseases, and that is next to the lung cancer. Depending on the epidemicology, cell scanning tests and animal verification, it is primarily confirmed that EGCG could act as the prevention and treatment from prostate cancer. Copper ion is necessary for the normal growth of mankind. After exceeding or lacking of copper ion in the body, it could result in many kinds of diseases including cancer. Recently, it has been becoming the focusing point for the scientists to investigate the interactions of metal ions with catechins extracted from green tea in the field of tea biochemistry and inorganic biochemistry. However, up to now it has been unclear in the world how to interact between copper ion and EGCG, and their behavior in cell biology.In this dissertation using PC-3 and LNCaP prostate cancer cells culture system, we investigated the effects of EGCG, Cu2+ and their coexistence on the growth of prostate cancer cell and their mechanism; the change of EGCG content in the cell culture system, and the dynamic of changes of chemiluminescence density of F-12 medium; the generation of free radicals in the presence of EGCG, copper ion or their coexistence.MTT assay demonstrated that EGCG or Cu2+ treatment of androgen-insensitive PC-3 and androgen-sensitive LNCaP PCA cells resulted in a maximum loss of cell viability at 24 h. When added EGCG after treatment with different concentrations of Cu2+, EGCG accelerated Cu2+-induced death of LNCaP cell unless the concentration ratio of EGCG/Cu2+ was enough high. However, EGCG inhibited the toxicity of Cu2+ to PC-3 cell in all groups. When Cu2+ were put into F-12 medium lastly, the toxicity of EGCG sharpened significantly in LNCaP cell culture system but in PC-3 cell, and EGCG-induced death of PC-3 cell was not accelerated unless the concentration of Cu2+ was enough high. Pretreated with EGCG, Cu2+-induced toxicity was decreased in LNCaP cell culture system. Cu2+-induced mortality of PC-3 cell was decreased in the presence of low concentrations of EGCG, and high concentrations of EGCG couldinhibit high concentrations of Cu2+-induced toxicity but low concentrations of Cu2+-induced toxicity to PC-3 cell. When EGCG was added into the system lastly, damages of LNCaP cell induced by Cu2+ were not inhibited unless the concentration ratio of EGCG to Cu2+ was enough high, and EGCG could not decreased Cu2+-induced toxicity in PC-3 cell culture system no matter what its concentrations. Also, results of flow cytometry and gel electrophoresis showed that necrosis was the main reason for inhibition of growth of PC-3 cell and LNCaP cell, and apoptosis was hardly detected. We found characters of necrosis and damages of cytoplasm membrane in PCA cells culture system, and cytoplasm membrane might be the important target point of EGCG, Cu2+ and their complex.EGCG content in F-12 medium with or without PC-3 cells was evaluated by HPLC. Without PC-3 cells, EGCG content of F-12 medium declined quickly at 37℃, 5.0%CO2. The change tendency of EGCG content was different in the presence or absence of PCA cells. EGCG was disappeared at 6h with PC-3 cells no matter what its concentration, and in a very small amount with LNCaP cells. We postulated that PCA cells might interact with EGCG Cu2+ could affect the change of EGCG content of F-12 medium, which was relative to added concentration and added order of Cu2+. EGCG disappeared at 6h with PCA cells in all groups, which probably had something to do with absorption of PC-3 to EGCG EGCG might exert its action in and out cells. Interaction of EGCG with Cu2+ was responsible for damages of cytoplasm membrane of PCA cells, and EGCG or its derivates might enter PCA cells and affect the growth of PCA cells. But derivates of EGCG, especially its complexs with metal ions, still were important components affected the growth of PCA cells.From chemiluminescence tests, chemiluminescence density of F-12 medium was increased after added EGCG or Cu2+,...
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