| Auxiliary liver transplantation (ALT), as an important proportion of LIVER TRANSPLANTATION, has been studied for more than 40 years, some experiences have been obtained in treating congenital hepatic diseases and acute liver failure. However, there are still some problems to be resolved, such as the long-term effect, establish the portal flow for prevent portal hypertension, especially the function competition between the graft and native liver and atrophy formation in graft. Upon to now, most of the animal experiments just concentrate on establishing the model and histology study, no further studies exploring the relationship between graft and native liver. This study establishes two patterns of heterotopic liver transplantations in rats with acute hepatic failure. By using the cell and molecular techniques, the relationship between native liver and grafts in these two models were investigated.METHODS:PARTI:According to the anastomosis pattern of portal flow, we establish twoheterotopic auxiliary liver transplantation models in normal rats, compare these two ALT patterns in some parameters; observe the effects of such factors as recipient rat status, surgical techniques, pre and postoperative managements on the survival rate of rats. The result demonstrates that these two models are easy to operate, and take less than 90 minutes to finish. The survival rate and time are steady; therefore, these two models are the ideal models to study further for ALT.PART 2:On the base of establishment of heterotopic liver transplantation, further study was carried out, the experimental animals were divided to 4 groups randomly, GROUP I : take as control group, just open-close operation; GROUP II: acute hepatic failure, ALF was induced by means of subtotal resection of the liver with only the quadrate lobe and right lateral lobe remaining; GROUP III: ALF + ALT + portal vein anastomosis with SMV; GROUP IV: ALF + ALT + portal vein arterializations. Observe the effects of such parameters as recipient rat status, hepatic function by serum laboratory examination, SPECT, histology study, ultrastructure study. In GROUP I , all the trials survived after operation, however, all of GROUP II were died in 48 hours after operation, in GROUP III and IV, 48-hour survival rate is 76.7%, 83.3% respectively, and after 24 hours, hepatic function by serum examination have a tendency to decent, although the result still higher than that of GROUP I . Before 15 days after operation, GROUP III and IV have no significantly difference in all parameters. However, after 15 days, compared with GROUP III, the graft of GROUP IV express degeneration, they have significantly difference in SPECT account.PART3:The group is same as PART 2, and we investigate apoptosis in graft by using DNA ladder, TUNEL and Apoptosis Index (AI). Meanwhile, the expressions of TRAIL and its receptor, STAT3 were also detected by RT-PCR technique. In terms of AI and expressions of TRAIL and its receptor, 30-day post-operation, Group IV have a significantly difference, compare with GROUP III; but in parameter of STATS, they have no difference.CONCLUSION1. Although the model of portal vein re-arterializations could not carry out by single person, two heterotopic liver transplantation models in rats were easy to operate, and the survival rate and time were steady.2. ALT, with or without portal vein re-arterializations, can improve the survival rate of acute liver failure rats3. With the time goes by, the function of the graft decent severely. At last the graft must be atrophy.4. In terms of Apoptosis and expressions of TRAIL and its receptor, compare with ALT, portal vein re-arterializations is more severely.5. The native liver of ALT without portal vein re-arterializations, since the portal flow was shunted, that makes it regenerates slowly. |
PDF Full Text Request