Study On The Effect And Mechanism Of MicroRNA - 466a - 3p On Allergic Rhinitis In Mice

Background:Allergic rhinitis is a common disease of Otolaryngology Head and Neck Surgery. It is IgE-mediated immune response associated with increased Th2cytokines secretion, eosinophils in the nasal mucosa and nasal secretions.Methods:By using microRNA microarray screening, nasal mucosa of mice with allergic rhinitis expressed different levels of microRNAs, comparing with mice in control group. We used using websites to predict targets of microRNAs, and we focused on the effect of mir-466a-3p which decreased in mice mucosa with allergic rhinitis, on the course of the Thl/Th2imbalance. Dual luciferase method was used to validate whether the predicted target gene GATA3is the target of mir-466a-3p. When mir-466a-3p was overexpressed, expression of protein GATA3decreased in mouse spleen mononuclear cells. Further, after the mice with allergic rhinitis were injected mir-466a-3p mimics through tail vein, the symptoms of allergic rhinitis, related indicators and protein GATA3were detected.Results:Results of microRNA microarray found that differences existed in microRNA between allergic rhinitis and normal mice. Meanwhile, in vitro experiments confirmed that mir-466a-3p can changed the expression of protein GATA3, and in vivo experiments found that elevating the expression of mir-466a-3p resulted in the downregulation of protein GATA3, which plays an important role of Thl/Th2balance.Conclusion:This study explored the role of microRNAs in allergic rhinitis, providing a new perspective for its pathogenesis, laid the theoretical basis and experimental reference for specific microRNAs as molecular targets for the treatment of allergic rhinitis. It has important theoretical and practical significance.