The Basic Relative Study On Molecular Biology Of Color Variation In Lycoris Longituba

The genus Lycoris have colorful flowers favorable for instance as Lycoris longituba. The flowers of L. longituba exhibit a great deal of diversity in both color and floral form, and its bulb has some medicinal potential.In this research, the Molecular Biology of Color Variation in L.longituba was studied. The main results were showed as following:1,The flower color of 43 L. longituba varieties and the Type species were divided into nine groups: red, orange, yellow and purple series.Anthocyanin composition of L. longituba determined using HPLC-DAD/ESI-MS. The result of multiple linear regression analysis (MLR) indicated that Cy derivatives were the major factors affected the value of color parameters greatly.2,Six complete-length cDNAs encoding ANS, CHS, DFR, F3H, F3'5'H and FLS were isolated from the petals by RACE.3,The spatial expression analysis of ANS, CHS, DFR, F3H, F3'5'H and FLS in L. longituba indicated that these genes were preferentially expressed in flowers stems and leaves. The temporal expression analysis showed that different expressed patterns in different color flowers.4,The function of ANS, CHS, DFR and F3'5'H gene was confirmed by Agrobacterium-mediated transformation of petunia.These results indicated that these genes normally expressed in the transformant.5,Based on more than thirty thousand ESTs sequenced in the flower tissue of Lycoris longituba, 349 putative TFs were predicted, and then validated by real time RT-PCR.6,Totally 809 SSRs distributed in 738 ESTs were mined out. These results provide a base for the development and future application of EST-SSR markers in genus Lycoris.7,Analysis of the level and patterns of gemomic DNA methylation in different ploidy Lycoris radiate by MSAP indicated that 3128 genetic loci were produced by 36 pairs of selective primers. However, the results implied that the DNA methylation levels were not closely associated with the autopolyploidy level in L.radiata.8,The nuclear DNA content in Lycoris exhibited significant differences from that of the standard (L. longiflorum). Each species group was allied based on genome size, chromosome number, flower color and leaf morphology; larger genome size was associated with a lower the chromosome number.