Molecular Phylogenetic Analysis Of Duck In China

China, as one of the largest duck producers in the world, is rich in duck genetic resources .and there are many local breeds scattered throughout the country. and there are many local breeds scattered throughout the country. Many populations of ducks were formed through migration, segregation, natural selection, and artificial breeding, which had particularly excellent characteristics of germplasm. Most of them are often maintained in small populations given their low to medium performance in egg production and growth rate. Being less productive, they are threatened by the commercial varieties utilized by large domestic and foreign breeding companies. There were few research works in duck microsatellite in domestics and overseas. To study the phyiogenetic relationships and evolution of local ducks in China, using the nine local duck breeds and one mallard (Anas platyrhynchos), 516 individual, have been surveyed and their blood samples collected. Then the genetic diversity of these breeds was analyzed by seventeen microsatellite markers. Possible divergence from Hardy-Weinberg expectations was determined. Gene heterozygosity per locus and population (H), mean number of alleles per locus, PIC, the average observed number of alleles and the effective number of alleles were calculated. The parameters Fit, Fst, and Fis, the coefficient of gene differentiation (Gst) were calculated. Bonferroni's procedures (Weir,1996) were applied when necessary. Results show, 17 microsatellites can amplify products with stable and clear bands. Of the 17 microsatellite loci, the number of alleles ranged from 1 to 14 per locus. There are 66 private alleles and 188 communion alleles. The average observed number of alleles is 7.24. The effective number of alleles is 4.1. The average heterozygosity value (H) and polymorphism information content (PIC) is 0.6896 and 0.6506 respectively, and there are 84.7% microsatellite with PIC above 0.5, and 15.2% with 0.25-0.5%, and 5.9% below 0.25. These suggest that microsatellite markers we selected can provide reliable...