| Low temperature is one of the natural factors that cause severe loss in the yield of agriculture and forest. Each year the loss of crops that is caused by low temperature amounts to thousands and millions dollars around the world. And how to reduce the loss arouses a general attention from the governments. Since molecular plant breeding can shorten the course of breeding cold resistance cultivar and make up the deficiency of traditional breeding technology, it is possible an efficient way to improve plant chilling or freezing tolerance. So many researches of molecular biology have recently been performed to try to improve plant cold resistance. However, the achievement about improving plant cold resistance by cold inducible gene is seldom reported. Besides the potential reasons of existing difficulty of gene transformation and the transgene silencing or inactivity, it is also because there are seldom qualitative genes interrelated with cold resistance and cold inducing genes are mainly from the pattern plants which annually grow, like Arabidopsis and so on. If more organisms, such as woody plants, were used to isolate novel gene, more dominant genes responsive to cold injury could be cloned.Chimonanthus praecox (L.) Link, a shrub originated from China, blossoms lordly in deep winter, possessing many advantages, such as resistance of coldness, drought, and secateur. Among them, chilling tolerance is its main characteristic. As long as the temperature isn't below -15°C, it can safely live through the winter. In our rearch, a cold acclimation gene (Cpcor413pm1) was cloned from Ch. praecox flower, and the bioinformatics, RT-PCR expression and ectopical expression in tobacco were analyzed. The main results are as follows:1 Cloning of Cpcor413pml from Ch. praecox flowerBased on the initial analysis of EST (Expressed Sequence Tag. EST) of cDNA library of Ch. praecox flower, and homology alignment of congenetic sequence (DW223061), we found that the...
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