Express Analysis Of Genes Derived From The Bark Of Chinese Fir(Cunninghamia Lanceolata. Lamb.)

Differential display genes derived from the bark of two natural mutants of Cunninghamia lanceolata var. "dugan" and Cunninghamia lanceolata var. "jurong 0" were analyzed using mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). The results showed: 1) Twenty-nine differential expressed fragments were screened and sequenced. Nine differential display transcrips matche to sequences of known or unknown function in GeneBank by Blastn (score>60). Functions involve: ribosomal protein, signal transferring, polyubiquitin, resistant protein, histidine-containing phosphotransfer protein and ATP synthase respectively.2) Blastx analysis showed that putative function were assigned 12 DD fragments: seven fragments were similar to that of bacteria and animals, function of them involved in membrane structure, cellular component, enzyme activity of metabolizing process and unknown protein; function forecast of 5 DD fragments came from plants which function were related to zinc finger protein,cf2-like protein,Glycerophosapokiester phosphodiesterase/kinase and catalase CAT-2.3) there were still eight fragments with no hit in the GenBank.Clones from cDNA library of Cunninghamia lanceolata var. jurong 0 bark tissue were selected randomly and sequenced from 5' end. 1,119 effective ESTs were grouped into 354 Unigenes. Blastx showed:1) the number of sequences with high and moderate similarity were 58 and 70 respectively, total of low similarity and no hit with GenBank was up to 63.8%;2) putative gene function were assigned to 5 Unigenes consisted of high expressed frequency ESTs, 25 consisted of moderate expressed frequency ESTs and 98 Unigenes consisted of low expressed frequency ESTs.3) gene function: annotated gene expressed with higher frequency in the bark library of Chinese fir involved: one group was genes of photosynthesis process including components of photosystem pathway, chlorophyll a/b-binding protein, oxygen evolving enhancer protein and energy transferring etc.; another group was resistant protein induced by environmental stress, such as Cu/Zu-superoxide dismutase, bZIP transcription factor, heat stock protein, cytochrome etc.. Besides, ARP (Auxin-Repressed Protein) with high expressed frequency may be related with branching and elongate of stem in Chinese fir.