| By using fluorescent-labeling-based automated DNA sequencing and microsatellite polymorphism analyzing techniques, of 30 microsatellite loci derived from domestic cat (Felts catus), 15 were proved polymorphic, reproducible and reliable in amplifying tiger genomic DNA. They were applied to study the genetic diversity in tiger captive population involving 3 subspecies, Siberian tiger (Pant he ra tigris altaica), Bengal tiger (P t. tigris) and South China tiger (P t. amoyensis). These 15 microsatellites include namely Fca043, Fca074, Fca077, Fca066, FcaO9O, FcalO7, Fca304, F53, F141, F42, Fca391, Fca453, F146, Fca441 and Fca4l, of which 7 loci, Fca043, Fca074, Fca077, Fca304, Fca453, Fca441 and F42 were sequenced to discover the repeating motif and repeating times. In the population analysis of 36 tigers by using 15 microsatellites, 4-13 alleles were found in each locus. The allelic frequency and genotypic frequency of 10 loci showed significant departure from Hardy-Weinberg Balance (a=0.01) indicating the disequilibrium of allelic frequency and genotypic frequency distribution. Heterozygosity was found between 0.4937-4)8412 at species level (im36), 0.3691-41.8003 in Siberian tiger (n20), 0.3872--0.8917 in Bengal tiger (n1 1) and 0.5341桹.8565 in South China tiger (n~5). Observed frequencies of lieterozygotes in each locus basically match the heterozygositv, except for a few loci whose heterozygote frequency is rather lower. Each subspecies o抶ns a few special alleles. Genetic distance among 3 subspecies calculated based on the data of 15 loci is: Siberian tiger-Bengal tiger 0.2449, Siberian tiger-South China tiger 0.0687 and South China tiger-Bengal tiger 0.1031. The matching probability between two unrelated individual at 15 loci is l0l1~~10.i8. P1 value in Father-child-mother set is between ]59824.94~883l296.70, correspondingly, RCP value is between 0.99999374-4)99999989. In the sets without mother抯 sample, P1 is between498.23?448029.34, correspondingly, RCP value is between 0.99799693?.99999931. The study also showed that no successful amplification was detected when applied primer sets of these 15 microsatellites to other species namely roe deer, sika deer, domestic dog, sheep and human DNA. It is suggested that the primers can be reliable in analyzing field samples and mixed or contaminated samples. Taking the example of the captive population of Xiongsen Bear and Tiger Village, this study suggested 4 countermeasures to manage the population and maintain genetic diversity: 1) Keep the independence ot subspecies strictly; 2) avoid inhrecding~ 3) prolong the generation leneth; 4) promote the genetic exchange among populations. As a potential significance, this study can also provide a practical technique route and basis for the microsatellite research in other large felines.
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