Study On Effective Composition Extracting And Microencapsulation Of Traditional Chinese Medicine

In this thesis, the traditional Chinese medicine was the main study object. The optimum methods to extract the high quality components were developed. The activity of the effective components extracted from the traditional Chinese medicine was studied. In addition, experiment studies on the microencapsulation of two drugs were carried out.We utilized methanol to extract the effective components of Rumex japonicus, which was a new method based on traditional solution separation. Most anthraquinone and its derivatives could be extracted with this method. We continued to isolate and purify the extraction with TLC separation and silica gel chromatography. Comparing the results, the latter is better. We isolated three types of anthraquinone derivatives. The infrared spectrum analysis of the three concentrated compounds identified their corresponding absorbance. HPLC revealed three types of anthraquinone derivatives: emodin, chrysophanol, and physcion. To detect bacteriostasis activity of the anthraquinone components, we designed bacteriostatic experiment. Candida Aibicans and accharomyces cerevisiae were selected as the target. The two anthraquinone derivatives extracted were detected. The minimum inhibition concentratin(mic) were calculated. This could prove that the anthraquinone and its derivatives from the Rumex had antibacterial activity.In this research,we studied the ethanol-alkali extraction method to of astragalus polysaccharides and Schisandra chinensis Baill. The optimum extraction technology was identified by single factor investigation. The extraction rate of ethanol-alkali extraction method is much higher than water extraction and alkali extraction method. According to vitro bioactivity experiment, the polysaccharides extracted by ethanol-alkali solvent had an strong inhibition effect on superoxide radical and hydroxyl radical. And the polysaccharides from Schisandra chinensis Baill had inhibition effect on some test bacteria according to inhibition experiment on vivo bacteria. Meanwhile, the effect of different solvents on the extraction of effective components from the cell wall tissues was studied by the histochemical methods, such as the bare-handed section, swelling ratio, paraffin section, IR spectrum and cell wall component analysis. The results showed that the ethanol-alkali solvent could increase the swelling ratio as well as the swelling speed. The effective components of cell tissues extracted by ethanol-alkali solvent becomes loose shown by the paraffin section. According to the IR spectrum analysis and the results of cell wall tissue component analysis, it was found that the ethanol-alkali solvent could decrease the contents of pectin and hemicellulose in the cell wall to make the wall broken, and therefore the effective components can be extracted easily by the solvent and the extraction rate was increased.Ginsenoside is the main drug effective composition and the substance basis of physiological activity on ginseng. In this research, Polylactic acid (PLA) microspheres were prepared by conventional solvent evaporation method and alabum was also first used as a modifier. The preparation method of PLA microspheres was selected by orthogonal designing experiments. The result shows that the microspheres we prepared have relatively uniform diameter, the optimum even diameters is about 2.0-3.0um, and the surface of the microspheres is smooth. We encapsulate gineenoside Rg3 using a biodegradable polylactide(PLA) microsphere. This process was studied by emulsion solvent evaporation method for enhancing solubility and stability of ginsenoside Rg3. The mean diameters of the prepared PLA microspheres containing Rg3 were 40μm. The rate of retention and the recovery rate were all high. Ginsenoside Rg3 release from microspheres was studied by HPLC and detected by UV. It was found that the drug release curve fitted with Model Heller-Baker best. Furthermore,gelatin microspheres were repared by using emulsion chemical-crossline technique. The mean diameters of the gelatin microspheres were 13.72μm.We encapsulated erythromycin using this gelatin microspheres. The mean diameters of this gelatin microspheres containing erythromycin were 15.17μm.The drug loading ratio and encapsulation efficiency were satisfied. The erythromycin-loaded gelatin microspheres showed good release profiles with a nearly constant release in vitro degradation studies.