| The safety and sanitation of food plastic packaging materials are important aspects of ensuring food safety. Plasticizer PAEs are widely used in food grade PVC film and the jeopardize of migration arousing people's attention. This paper studies the migration of Di-2-ethylhexyl phthalate (DEHP) existing in food grade PVC film. Firstly, migration of DEHP from food grade PVC film into water food simulants was studied; secondly, GC method of determining DEHP in meat was established; thirdly, the influence of temperature, time, fat content and pH on the migration of DEHP into meat was investigated; fourthly, migration of DEHP under UV radiation and microwave was worked over, lastly, the influence of cooking and frying on DEHP content in meat was studied. The paper affords major results as follows:1. The determining method of DEHP in food plastic packaging materials was established by GC. The results showed that solvent was diethyl ether, soxhlet extracting 4h, the recovery rate lied between 90.7% and 105.1% and the detection limit was 6.76×10-4μg/ml in solution and 3.38×10-3mg/kg in sample.2. DEHP could migrate into all water food simulants from PVC film. (1) Trichloromethane was the best extracting solvent of DEHP in water food simulants compared with diethyl ether and hexane; (2) The sequence of DEHP migration rate in water food simulants was: 65% ethanol>20% ethanol>water>4% acetic acid; (3) Under different circumstances, range of DEHP migration rate was 0.19%～0.38%(water), 0.19%～0.45%(20% ethanol), 0.72%～6.32%(65% ethanol), respectively.3. The determining method of DEHP in meat was established by saponification and distillation. The technologic parameters were: extracted 6h by diethyl ether firstly, saponified 3h secondly, thus DEHP decomposed to phthalic acid and 2-ethyl-1-hexanol. 2-Ethyl-1-hexanol was determined after being steam distilled and the amount of DEHP was calculated by conversion. The recovery rate of the method lied between 75.12% and 84.95%, detection limit was 1.07×10-3mg/kg.4. Amount of migration of DEHP from PVC film into meat was influenced by temperature, time, fat content and pH value. (1) Amount of migration of DEHP increased with the time prolonging and temperature increasing. The amount of migration of DEHP was 1961.92mg/kg (75.12mg/dm2, 90℃, film/meat contacting 0.5h); (2). Amount of migration of DEHP increased with fat content adding. After film/meat contacting 60h, the amount of migration of DEHP in 45.2% samples reached 2255.91mg/kg (55.68mg/dm2, 20℃); (3) The amount of migration of DEHP was the highest in pH6 samples before meat degenerative and the top value was 1281.59 mg/kg (49.00mg/dm2, film/meat contacting 48h, 20℃).5. Ultraviolet radiation and microwave could enhance the migration of DEHP from PVC film into meat. (1) After Ultraviolet radiating 12h, the amount of migration of DEHP was 39.55% higher than control samples; (2) The average amount of migration of DEHP was 534.92mg/kg (13.76mg/dm2) after microwave heating 16s, 850W, while the control samples under detection.6. The amount of DEHP in contaminated samples significantly decreased after cooking and frying. (1) After cooking 30min, the amount of DEHP was lower 50.96%, from 536.80mg/kg to 263.26mg/kg before and after cooking; (2) After frying 3min, the amount of DEHP was lower 61.49%, from 536.80mg/kg to 206.70mg/kg before and after frying.7. Amount of migration of DEHP differed in different foods. (1) The recovery rate of DEHP saponification and distillation method ranged from 56.39% to 97.40% in different foods; (2) The picks out rate of DEHP was 68.75% in 16 samples after film/meat contacting 24h(20℃) . The range of amount of migration of DEHP was 635.97mg/kg (17.71mg/dm2)～892.03mg/kg (25.58mg/dm2) in picked out samples.8. The innovation of the paper was as follows: (1) The determining method of DEHP in meat was established by saponification and distillation for the first time; (2) The determining method of DEHP in food plastic packaging materials was established by GC and soxhlet extracted; (3) The influence of temperature, time, fat content and pH on the migration of DEHP from PVC film into meat was successfully researched by using the model of Colorimetric tube of film/meat contacting for the first time.The paper affords cheap and rapid determining methods and amount of migration of DEHP under different contacting conditions for researching the migration of DEHP from food grade PVC film into foods and offers strongly instrument for ensuring the safety of food packaging.
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