| Natural phospholipids are recognized as bioactive substances with relatively valuable for pharmaceutical, because they exhibit many important physiological activities. In addition, they are also ideal natural surface-active agents and have been used in the fields of pharmaceutical, nourishment and cosmetic industries. The high purity fraction phospholipids have been used in above industries, especially in pharmaceutical. With the research and application of liposome formed by phospholipids to be used as carriers for anti-cancer and gene-curing drugs increase recently, the demand for high purity fraction phospholipids enhances greatly. However, the high purity fraction phospholipids are expensive at present, which restricts the phospholipids to be applied largely in the field of pharmaceutical. It is very difficult to separate and purify the natural phospholipids for obtaining the high purity fraction phospholipids, which lead their high prices. Amongst the fraction phospholipids, the other phospholipids are separated by HPLC except the phosphatidylcholine to be purified by using simple column chromatography. Owing to the elution mode to be used in HPLC, in which there are some inherent shortcomings, i.e., low loading amount, high solvent consumption, low concentration of product and significant tailing of the peaks, it leads to high production cost and price. How to reduce the production cost is a problem to need solving. It is also a vital problem that whether the high purity phospholipids can meet the demand of development of pharmaceutical or not.Aiming at the actuality of the utilization of the phospholipids, the phospholipids are separated by using column chromatography with simple eluent system and high performance displacement chromatography (HPDC) for reducing the cost. In order to exploit and extend the application of the phospholipids in the field of pharmaceutical, the research of screening drugs by using liposome chromatography with magnesia-zirconia coated natural phospholipids as the stationary phases is achieved. The main contents studied are as follows:1. The methods of qualitative and quantitative analysis of the phospholipids are established. TLC is a high efficient and fast method for qualitative analysis owing to simple and fast operation and several samples to be developed simultaneously. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidic acid (PA) are separated completely on a2.5cm X 7.5cm silica G plate by using chloroform-methanol-water (65:25:4, V/V) as the developer. HPLC has some advantages such as high precision and detection on-line. The good separation of five phospholipids is obtained on a 150mmx4.6mm silica column by using acetonitrile-methanol-85% phosphoric acid (180:3:1, v/v) as the mobile phase at the flow-rate of 0.5ml/min. From the high recovery and repeatability of the retention time and peak height, it can be concluded that HPLC exhibits high precision for qualitative analysis of five phospholipids and quantitative analysis of PC, PE and PI.2. Separation and purification of PC and PE from soybean oil degummed residues by using solvent extraction and column chromatography has been studied. The water and oil are removed from soybean oil degummed residues, and then separated into two parts (crude PC and PE) by using 95% ethanol extraction. The crude PC and PE are used as the raw materials for chromatography separation on a silica column, respectively. The PC product (purity>90%) can be obtained when the crude PC is isocraticly eluted by methanol. The PE product (purity>75%) can be obtained when the crude PE is eluted by using the gradient mode of chloroform-methanol (2:1, V/V) to methanol.3. Separation of a binary mixture of PC and PE by using HPDC has been studied for the first time. The binary mixture of PC and PE is successfully separated on an 150mmx4.6mm analytical silica column (3-5 w m packing), using dichloromethane-methanol (9:1, V/V) as carrier and ethanolamine as displacer which...
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