Design, Synthesis, Characterization And Cell-Imaging Of Fluorescence Magnetic Nanoparticles And Fluorescence Probe For Metal Ions

In recent years, with the continuous development of life science, people active species within cells, cell signal transduction and apoptosis and other aspects of more and more depth. Fluorescence imaging analysis is now widely used in the analysis of living cells is an effective visual analysis techniques. Currently used for live cell imaging analysis of fluorescent probes and more for the organic fluorescent dye molecules, which for the analysis of living cells provides an effective fluorescent probe. In practice, the existence of organic fluorescent dye molecule fluorescence lifetime is shorter, easier and instability quenching defects. Therefore, to achieve long continuous measurement and real-time dynamic visual analysis to improve the selectivity and accuracy is an urgent need to address the problem. Metal complexes with a fluorescence lifetime of fluorescent probes as long, narrow emission peaks and Luminescence Stokes shift and large, has aroused attention and concern. However, due to metal complexes as fluorescent probes less than the general solubility, functional relatively simple, and slightly toxic and other shortcomings. In this paper we use has a unique magnetic and chemical properties of the magnetic iron oxide as the carrier of a new fluorescent probe, designed and synthesized a number of metal complexes based on nano-magnetic fluorescent probe, both long and stable fluorescence imaging, but also magnetic resonance imaging.To this end the paper includes the following four areas:1. Reviewed a variety of fluorescent probes and research status and as a fluorescent probe vector preparation and application of nano-particles of the situation.2. When the ruthenium complex combination of direct and when Fe3O4 nanoparticles, Fe3O4 will quench its fluorescence. Therefore, Fe3O4 coated with a water-soluble polymer to nanoparticles. At the same time the length of the polymer chain of the degree of quenching. Found that when the polymer molecular weight of 1900 is the best length. Synthetic vectors based on the transition magnetic Fe3O4 nanoparticles of ruthenium metal, the solubility of the ruthenium complex from 10.6μg Ru/ml to 23.6 Ru/ml, the solubility increased by almost 1-fold, while the solubility calculated based on each of Fe3O4 nanoparticles Approximately 36 ruthenium complexes with molecules. This synthesis of magnetic nanoparticles and fluorescence relative stability of traditional fluorescence probe, is not easy to quench, for the analysis of living cells provides an effective method and means of fluorescence.3. Under the guidance of the above ideas are synthetic vector based on Fe3O4 magnetic nanoparticles rare earth metal Eu. Also developed a "one pot" to quickly and accurately coated magnetic Fe3O4 nanoparticles. By IR, UV, fluorescence, electron microscopy, magnetic measurements, dynamic light scattering nanoparticles were characterized. The magnetic nanoparticles can all be well on the cell fluorescence imaging, and by 3D laser scanning confocal microscopy has been confirmed. At the same time by magnetic resonance imaging of the nano-materials can be a good T2 imaging. Therefore, the synthesis of the work of fluorescent magnetic nanoparticles is a good multi-functional imaging reagents.4. Use of "on-off"fluorescent molecular switch principle, structure design and synthesis of the two different rhodamine-based fluorescent probes 1,2 and 3. UV absorption and fluorescence spectra of the test results show that 1 and 2 to select the identification of high mercury ions and copper ions. And binding ratio of 1 and Hg2+, 2 and Cu2+were 2 and 1, respectively. Fluorescent probes 1 and 2, respectively, Hg2+ and Cu2+on the detection limit can reach ppb levels, and these two metal ions to the naked eye detection. This will be in the actual production and life has a very wide range of applications. Also confirmed by laser confocal fluorescence microscope probes 1 and 2 have good ability for imaging Hg2+and Cu2+...